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Primary human tumor models are established
by transplanting fresh human tumor fragments onto immune-deficient
mice. Compared with cell line based traditional xenograft
models, primary models better mimic original patient histopathology,
as well as gene expression profile, therefore can provide
better predictive value for cancer indication prioritization
and clinical efficacy estimation. CrownBio has one of the
largest collections of primary human tumor models, including
gastric, colorectal, liver, lung, H&N, and kidney cancer
models.
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Living TissueTM Bank :
Is a collection
of hundreds of cryopreserved human tumor tissue ex-plants,
which still retain the ability to engraft immune-deficient
mice upon reviving. We have kept detailed clinical information
about each tissue specimen, such as patient prior treatment
history, original and serial in vivo passage histopathology,
as well as cDNA expression profiles. Further characterization
such as DNA mutation and copy number can also be obtained
upon request.
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Efficacy
study with human primary tumor models :
tumors are established
using cryo-preserved Living Tissue fragments. Mice are
randomized into different dosing groups with 10-12 mice
in each group when the average tumor size reaches 100-200mm3.
In order to obtain statistical power, we recommend testing
candidate drugs on multiple primary tumor models, either
with the same cancer type to estimate efficacy, or among
different cancer types to gain predictive information
on potential clinical indications.
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