AACR-NCI-EORTC 2023 Poster LB_B16

Quantification of Immuno-oncology Drug Responses in Autologous Co-cultures of Ex Vivo Patient Tumor Tissues with PBMCs

Niels Meesters, Sander Basten, Ezgi Kaya Aksoy, Mariusz Madej, Kuan Yan, Emma Spanjaard, Leo Price, and Nataliia Beztsinna

Crown Bioscience Netherlands, J.H. Oortweg 21, 2333 CH Leiden, The Netherlands

This research presents a study aimed at further boosting the immune compartment of the 3D Ex Vivo Patient Tissue (EVPT) platform assay by addition of stained autologous immune cells (PBMCs).

The 3D Ex Vivo Patient Tissue (EVPT) platform combines short-term 3D ex vivo tumor culture systems from fresh human material with high content imaging analysis. The platform preserves the innate tumor microenvironment, including resident immune cells, and thus allows immunological testing in ‘close-to-patient’ scenario.

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• 3D patient tissue workflow including tissue collection and preparation, 3D imaging analysis, and phenotypic readouts
  
  
• Sample sensitivity towards immune therapies and the baseline killing effect of autologous PBMCs
  
  
• Quantified drug effects on tumoroids and PBMCs

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