ENA21 Poster 113

Image-Based Quantification of Immunotherapeutic Effect on the Tumor-Immune Interactions in 3D Co-Cultures

Lidia Daszkiewicz, Gera Goverse, Nataliia Beztsinna, Marjan van de Merbel, Benjamin Visser, Tomas Veenendaal, Emma Spanjaard, Kuan Yuan, Leo Price
Crown Bioscience Netherlands B.V., Leiden, The Netherlands

Despite the increasing number of immunotherapies available in preclinical cancer research and drug discovery pipelines, the number of patients benefiting from these immunotherapies remains extremely low. Moreover, the failure of immunotherapies in clinical trials remains very high to date. These high failure rates could be attributed to the lack of deep understanding of the mechanism of action of drug candidates and to the absence of clinically relevant preclinical models that are suitable for drug screening purposes.

In this poster we address these issues, by detailing our in vitro drug screening platform, which is suitable for high-throughput testing in a physiologically relevant 3D environment. Our preclinical drug testing platform is based on the co-culture of tumor cells with immune cells in 3D. Image analysis is applied to read out effects of drug candidates on immune cell migration, immune cell infiltration and tumoroid killing.

Download this Poster to Discover:

• How our 3D in vitro system based on the co-culture of tumor cells and immune cells allows high-throughput visualization and quantification of immunotherapy effects in a more physiologically relevant setting, compared to traditional 2D cultures
  
  
• How this platform can be applied to dissect the effects of immunotherapy on different cell populations

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