Huajun Yang, Zhongliang Li, Beibei Tang, Phillip Wang, Qinyun Ma, Frank Xing, and Qian Shi
Macrophages provide an important surveillance system, which can be escaped by human tumors expressing CD47. Reinstating macrophage functionality for clearing tumor cells, by targeting CD47 and thereby blocking the phagocytosis inhibitory CD47-SIRPalpha interaction, has therefore become an important strategy in immunotherapy.
CrownBio has developed a robust and reproducible in vitro assay platform to support preclinical anti-CD47 drug research. The technique applies a macrophage differentiation system using isolated CD14+ monocytes as starting material, and is able to achieve greater than 80% of M1 and M2 populations throughout a 6 day differentiation period.
This poster details initial in vitro findings, as well as validating the data in vivo via paired isogenic cell models in which the CD47 gene is knocked out in one sub-cell line and overexpressed in a second sub-cell line.
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2024-01-16
2021-10-28
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