Poster B209: PDX as Surrogate Models for TME Investigation

Transcriptomic Analysis of Patient-Derived Xenografts Reveals Heterogeneity in Human and Mouse Stroma/Immune Compartments

Jia Xue, Wubin Qian, Sheng Guo, Jie Cai, Davy Ouyang, and Henry Q. Li

It is being increasingly recognized that cancers are collections of diverse immune, as well as genetic, diseases. Aspects such as the tumor microenvironment, and its immune components, play a critical role on tumor progression and response, and need to be fully investigated.

Studying stroma-specific components has been historically challenging; however, newer experimental animal models can provide excellent systems for investigating the TME. For example patient-derived xenograft (PDX) models allow for straightforward in silico separation of human and mouse content, providing a surrogate model for TME and tumor-stroma interaction studies.

CrownBio provides the world’s largest commercial collection of PDX models, and is completing whole transcriptome sequencing of this model panel. Downstream analyses are allowing investigation of human and mouse stroma and immune compartments, which are reviewed in this poster.

Read this Poster to Discover:

• Whole transcriptome sequencing of almost 1,500 PDX models, with mouse-to-human sequencing read ratio consistent with previous reports
• The identification of all types of adaptive and innate immune cells in mouse stroma and a variety of human immune components, with levels varying across indications and individual models
• How cross-species correlation networks identified novel relationships between human and mouse genes across all PDXs and in specific cancers

Download the Poster Now!