SITC 2022 Poster 69

A High Content Screening Platform for Testing Immunotherapies in Patient-Derived Organoids

Saskia de Man, Tomas Veenendaal, Michelle Kop, Nataliia Beztsinna, Daniel Okkes, Ashgard Weterings, Leo Price, Gera Goverse

In this study a high throughput screening platform is presented, which allows testing of cancer immunotherapies in co-cultures of human organoids with immune cells. Incorporating diverse cellular players from the immune system and stromal compartment, such as cancer associated fibroblasts (CAFs), allows the reconstitution of complex cellular interactions that occur in the tumor micro-environment (TME).

Our proprietary, automated high content imaging (HCI)-based analysis provides visualization and quantification of immune cell-mediated effects in this complex, physiological relevant 3D screening platform. Functional readouts such as migration of immune cells towards organoids, infiltration into the organoids and their killing are obtained, allowing for a better understanding of the immune-modulatory profile of immuno-oncology.

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• Differences in the sensitivity and kinetics of organoid models from the same indication and from different indications towards the killing by immune cells.
  
  
• Pre-activation or reactivation of immune cells within the 3D co-cultures.
  
  
• Quantitative readouts obtained by testing cellular therapy of engineered T cells in co-culture with CRC organoids.

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