New Publication

An NGS-based assay for accurate detection and quantification of immune gene expression in mouse tumor models

Authors: Jia Xue, Xiaobo Chen, Xiaoyu An, Jingjing Wang, Mingfa Zang, Binchen Mao, Sheng Guo, Tao Yang, Rajendra Kumari, Qi-Xiang Li

Crown Bioscience, Inc., San Diego, California, United States of America

Here, we introduce a mouse immune-oncology NGS-based (NGSmIO) assay for accurately detecting and quantifying the mRNA expression of 1080 tumor microenvironment (TME) related genes in mouse tumor models. The NGSmIO panel was shown to be superior to the commonly used microarray approach by hosting 300 more relevant genes to better characterize various lineage of immune cells, exhibits improved mRNA and protein expression correlation to flow cytometry, shows stronger correlation with mRNA expression than RNAseq with 10x higher sequencing depth, and demonstrates higher sensitivity in measuring low-expressed genes.

Download our paper to discover the advantages this targeted RNA-Seq panel brings to your immune-oncology research.