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In Vitro

Boost oncology drug discovery with XenoBase®, featuring the largest cell line selection and exclusive 3D organoid models. Benefit from OrganoidXplore™ and OmniScreen™ for rapid, in-depth analysis.

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In Vivo

Enhance drug development with our validated in vivo models, in vitro/ex vivo assays, and in silico modeling. Tailored solutions to optimize your candidates.

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Tissue

Experience ISO-certified biobanking quality. Access top biospecimens from a global clinical network, annotated by experts for precise research.

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Biomarkers and Bioanalysis

Leverage our global labs and 150+ scientists for fast, tailored project execution. Benefit from our expertise, cutting-edge tech, and validated workflows for reliable data outcomes.

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Data Science and Bioinformatics

Harness your data and discover biomarkers with our top bioinformatics expertise. Maximize data value and gain critical insights to accelerate drug discovery and elevate projects.

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KRAS

Accelerate innovative cancer treatments with our advanced models and precise drug screening for KRAS mutations, efficiently turning insights into clinical breakthroughs.

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EGFR

Advance translational pharmacology with our diverse pre-clinical models, robust assays, and data science-driven biomarker analysis, multi-omics, and spatial biology.

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Drug Resistance

Our suite integrates preclinical solutions, bioanalytical read-outs, and multi-omics to uncover drug resistance markers and expedite discovery with our unique four-step strategy.

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Patient Tissue

Enhance treatments with our human tumor and mouse models, including xenografts and organoids, for accurate cancer biology representation.

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Bioinformatics

Apply the most appropriate in silico framework to your pharmacology data or historical datasets to elevate your study design and analysis, and to improve your chances of clinical success.

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Biomarker Analysis

Integrate advanced statistics into your drug development projects to gain significant biological insight into your therapeutic candidate, with our expert team of bioinformaticians.

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CRISPR/Cas9

Accelerate your discoveries with our reliable CRISPR solutions. Our global CRISPR licenses cover an integrated drug discovery platform for in vitro and in vivo efficacy studies.

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Genomics

Rely on our experienced genomics services to deliver high quality, interpretable results using highly sensitive PCR-based, real-time PCR, and NGS technologies and advanced data analytics.

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In Vitro High Content Imaging

Gain more insights into tumor growth and disease progression by leveraging our 2D and 3D fluorescence optical imaging.

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Mass Spectrometry-based Proteomics

Next-generation ion mobility mass spectrometry (MS)-based proteomics services available globally to help meet your study needs.

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Ex Vivo Patient Tissue

Gain better insight into the phenotypic response of your therapeutic candidate in organoids and ex vivo patient tissue.

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Spatial Multi-Omics Analysis

Certified CRO services with NanoString GeoMx Digital Spatial Profiling.

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Biomarker Discovery

De-risk your drug development with early identification of candidate biomarkers and utilize our biomarker discovery services to optimize clinical trial design.

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DMPK Services

Rapidly evaluate your molecule’s pharmaceutical and safety properties with our in vivo drug metabolism and pharmacokinetic (DMPK) services to select the most robust drug formulations.

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Efficacy Testing

Explore how the novel HuGEMM™ and HuCELL™ platforms can assess the efficacy of your molecule and accelerate your immuno-oncology drug discovery programs.

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Laboratory Services

Employ cutting-edge multi-omics methods to obtain accurate and comprehensive data for optimal data-based decisions.

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Pharmacology & Bioanalytical Services

Leverage our suite of structural biology services including, recombinant protein expression and protein crystallography, and target validation services including RNAi.

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Screens

Find the most appropriate screen to accelerate your drug development: discover in vivo screens with MuScreen™ and in vitro cell line screening with OmniScreen™.

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Toxicology

Carry out safety pharmacology studies as standalone assessments or embedded within our overall toxicological profiling to assess cardiovascular, metabolic and renal/urinary systems.

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Our Company

Global CRO in California, USA offering preclinical and translational oncology platforms with high-quality in vivo, in vitro, and ex vivo models.

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Background Image

Next Generation Mass Spectrometry-based Proteomics Services

The Power of Ion Mobility and Data Independent Acquisition Strategy

Crown Bioscience offers next-generation ion mobility mass spectrometry (MS)-based proteomics services to global clients.

This will allow clients to assess the biological function of proteins for understanding signaling mechanisms within cells as well as specific biomarkers to diseases, and systematically assess quantitative differences in protein profiles of distinct samples for biomedical and clinical research.

Services Offered

protein post translational modifications-teal

Post-translational
modification analysis

Post-translational modification analysis and bundle service options are available. Please contact us for details.

Applications:

  • Global proteomics profiling of cells or tissues, with/without treatment
  • Proteomics biomarker discovery and validation
  • Drug mechanism of action and toxicity studies
  • Disease mechanism studies
  • Target identification and validation
  • Complementary analysis and correlation approaches for other omics analysis

Our Advantages

  • Better reproducibility:
    No missing values, reduced batch effect, better data parallelism, and traceability
  • Increased sensitivity and wider dynamic range, improved detection depth:
    Identify more proteins from same or lower amount of sample, significantly improved quantification especially for low abundant proteins

  • Lower amount of samples required

  • Suitable for large sample cohort studies

  • Proteomic biomarker discovery service
    Customized bioinformatic analysis available for large cohort studies

Why proteomics is important

 

Most diseases are manifested at the level of protein activity. Proteomics seeks to directly correlate the involvement of specific proteins, protein complexes and their modification status in disease states. The level of transcription of a gene gives only a rough estimate of its level of expression into a protein, and moreover, it cannot reflect the protein post transcription modifications which profoundly affect their activities.

Liquid chromatography (LC) coupled with mass spectrometry (MS) has become the gold standard in various omics fields. However, the proteome is highly complex with a wide dynamic range, and the coverage of proteomes in complex biological samples remains challenging due to the limited speed, sensitivity, and resolution of current mass spectrometers.

Factors to consider when choosing MS-based proteomics services

 

Liquid chromatography (LC) coupled with mass spectrometry has become the gold standard in various omics fields. When choosing MS-based proteomic services, there are several factors to consider:

  • Data completeness and reproducibility
  • Sensitivity and identification depth:
    • Number of identified and quantified proteins
    • Low abundant protein identification and quantification
    • Sample amount requirement
  • Speed, throughput, robustness and cost
  • Data analysis

State-of-Art Mass Spectrometry Core Facility

  • 20 top mass spectrometers such as Bruker timsTOF Pro/ timsTOF Pro 2 series, and Thermo Fisher Scientific Orbitrap Exploris™ 480
  • Additional separation dimension by ion mobility separation to traditional LC-MS/MS based proteomics
    • Bruker Trapped Ion Mobility Spectrometry (TIMS)
    • Thermo Fisher Scientific FAIMS
  • Unique diaPASEF technology to dramatically increase data acquisition speed
  • Evosep One provides robust and excellent chromatographic separation
  • Continuous analysis of thousands of samples to provide stable performance without cleaning, enhancing the stability of batch analysis

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